Saturday, June 21, 2025

PATHOLOGY LAB ASSISTANT

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Mode: Offline

PATHOLOGY LAB ASSISTANT

Three Months Job Oriented Training


Mode: Offline


100% Placement Assistance


Hurry up! Limited Seats Book Now


Training Venue: ITLS Academy, Lucknow


Hostel Facility Available


Registration Link: https://itlsacademy.com/registration.php?program=133&courses=949


More detail: https://itlsacademy.com/courses.php?cid=T1RRNQ==


Pay Now: https://itlsacademy.com/page.php?mid=TlRNPQ==

After fee payment please send screenshot on whatsapp at 7080833450

Friday, June 20, 2025

Food Processing, Three Months Online Certificate Course


Mode: Online

FOOD PROCESSING

Three Months Online Certificate Course

Eligibility: Any Biological Science, Agriculture & Pharmacy Candidate

Job Prospects: Food Industry

TOPICS TO BE COVERED

PART I BASIC PRINCIPLES

1 Properties of foods and processing theory

2 Process control

PART II AMBIENT-TEMPERATURE PROCESSING

3 Raw material preparation

4 Size reduction

5 Mixing and forming

6 Separation and concentration of food components

7 Fermentation and enzyme technology

8 Irradiation

9 Processing using electric fields, high hydrostatic pressure, light or ultrasound

PART III PROCESSING BY APPLICATION OF HEAT

A.    Heat processing using steam or water

10 Blanching

11 Pasteurisation

12 Heat sterilization

13 Evaporation and distillation

14 Extrusion

B.     Heat processing using hot air

15 Dehydration

16 Baking and roasting

C.    Heat processing using hot oils

17 Frying

D.    Heat processing by direct and radiated energy

18 Dielectric, ohmic and infrared heating

PART IV PROCESSING BY THE REMOVAL OF HEAT

19 Chilling

20 Controlled- or modified-atmosphere storage and packaging

21 Freezing

22 Freeze drying and freeze concentration

PART V POST-PROCESSING OPERATIONS

23 Coating or enrobing

24 Packaging

25 Filling and sealing of containers

26 Materials handling, storage and distribution

Gene Cloning Online Training


Mode: Online

GENE CLONING

Two Months Online Training

Eligibility: Any Biological Science, Agriculture, Pharmacy, Medical Science candidate can join

TOPICS TO BE COVERED

Part I THE BASIC PRINCIPLES OF GENE CLONING AND DNA ANALYSIS

1.      Why Gene Cloning and DNA Analysis are Important

2.      Vectors for Gene Cloning: Plasmids and Bacteriophages

3.      Purification of DNA from Living Cells

4.      Manipulation of Purified DNA

5.      Introduction of DNA into Living Cells

6.      Cloning Vectors for E. coli

7.      Cloning Vectors for Eukaryotes

8.      How to Obtain a Clone of a Specific Gene

9.      The Polymerase Chain Reaction

PART II THE APPLICATIONS OF GENE CLONING & DNA ANALYSIS IN RESEARCH

10.  Sequencing Genes and Genomes

11.  Studying Gene Expression and Function

12.  Studying Genomes

PART III THE APPLICATIONS OF GENE CLONING AND DNA ANALYSIS IN BIOTECHNOLOGY

13.  Production of Protein from Cloned Genes

14.  Gene Cloning and DNA Analysis in Medicine

15.  Gene Cloning and DNA Analysis in Agriculture

16.  Gene Cloning and DNA Analysis in Forensic Science and Archaeology

Biotech Internship on Molecular Artificial Intelligence

 



Mode: Online

MOLECULAR ARTIFICIAL INTELLIGENCE (AI)

One Month Online Training

Key Features: Recorded Lectures, Practices on biological (tools) software, Training Report, Online Test

Certificate will be provided

TOPICS & TOOLS (SOFTWARE) TO BE COVERED

WEEK 1: INTRODUCTION TO MOLECULAR AI

Goal: Understand basic concepts of Molecular Biology, AI, and their intersection.

๐Ÿ”ฌ Molecular Biology Essentials

  • Central Dogma (DNA → RNA → Protein)
  • Gene structure and regulation
  • Primer Designing, sequencing, In-silico electrophoresis

Artificial Intelligence Basics

  • Introduction to AI, ML and DL
  • Types of Learning: Supervised, Unsupervised, Reinforcement
  • Concepts: Algorithms, Neural Networks, Decision Trees

WEEK 2: BIOINFORMATICS TOOLS FOR GENOMICS

Goal: Learn how AI supports genomics using bioinformatics tools.

๐Ÿ”ง Tools:

  • NCBI, Protein Ensembl
  • BLAST, mmseq2 (sequence alignment)
  • Geneid , Genscan
  • PSA, MSA
  • RNA Secondary Structure Prediction

WEEK 3: PROTEOMICS AND STRUCTURE PREDICTION

Goal: Apply AI tools in protein structure, function, and interaction.

๐Ÿ”ง Tools:

  • UniProt, PDB, Swiss-Model, AlphaFold
  • STRING, Cytoscape (for protein interaction networks)
  • Protein Visualization

WEEK 4: AI IN DRUG DISCOVERY AND SYSTEMS BIOLOGY

Goal: Explore AI-based drug design and integrative biology.

๐Ÿ”ง Tools:

  • CBDock, PyRx (Molecular docking)
  • PubChem, DrugBank, KEGG
  • Enzyme, Brenda

How to get biotech job?

 

Start your success journey now…

BIOTECHNOLOGY

BOOK APPOINTMENT NOW

Who can join? Any Biotechnology candidate (confused about Biotechnology scope/career) can join

HIGHLIGHTS

1.     ONE TO ONE DISCUSSION

2.     Parents can also join with candidate

3.     Candidate can ask their doubts about career

a.     How to start career in industries

b.     Guidance for higher studies

4.     Candidate will know about their common mistakes so they are not able to start good career

5.     Scope in India & abroad

6.     Don’t waste your time & money with fake job consultancies

7.     How to improve yourself?

8.     How to choose industry/Company?

9.  Starting salary packages 

Thursday, June 19, 2025

Enzymology Training

 


Hurry up! Book Your Seat Now

Mode: Offline/Online

๐Ÿงช Enzyme Analysis Techniques – Overview

Enzyme analysis techniques are essential for studying enzyme activity, kinetics, specificity, inhibition, and stability. These methods are widely used in biotechnology, food science, pharmaceuticals, diagnostics, and clinical biochemistry.


๐Ÿ” 1. Enzyme Activity Assays

Colorimetric Assays

  • Measure color change due to enzyme action.

  • Example: Amylase breaking down starch (iodine test), peroxidase with guaiacol.

  • Spectrophotometer used to measure absorbance.

Fluorometric Assays

  • Use fluorescent substrates; very sensitive.

  • Common in diagnostic and clinical assays (e.g., protease activity).

Turbidimetric Assays

  • Measure reduction in turbidity (cloudiness) due to enzymatic action (e.g., lysozyme breaking bacterial cell walls).

Titrimetric Assays

  • Involve titration to measure product or substrate concentrations.

  • Example: Lipase activity via free fatty acid titration.


๐Ÿงฌ 2. Enzyme Kinetics

  • Michaelis-Menten kinetics: Determines Km (substrate affinity) and Vmax (max activity).

  • Tools: Lineweaver-Burk, Eadie-Hofstee plots.

  • Helps in understanding catalytic efficiency and comparing enzyme variants.


๐Ÿšซ 3. Enzyme Inhibition Studies

  • Types: Competitive, Non-competitive, Uncompetitive

  • Measure how inhibitors affect enzyme activity.

  • Important for drug discovery and therapeutic enzyme design.


๐ŸงŠ 4. Enzyme Stability Tests

  • Temperature and pH stability profiles.

  • Thermal inactivation curves (e.g., T₅₀ or half-life).

  • Storage and shelf-life testing for industrial enzymes.


๐Ÿงช 5. Protein Estimation & Enzyme Purity

  • Bradford Assay, Lowry Method, or BCA Assay for total protein.

  • SDS-PAGE to check enzyme purity.

  • Specific activity = (Enzyme activity per mg of total protein)


๐Ÿงฐ 6. Modern & Instrument-Based Techniques

TechniquePurposeExample
SpectrophotometryAbsorbance-based activityNADH oxidation (340 nm)
FluorometryLow-concentration detectionProtease or phosphatase assays
HPLC/UPLCSeparation of substrates/productsEnzyme kinetics
Mass Spectrometry (MS)Enzyme identification & modificationProteomics
Isothermal Titration Calorimetry (ITC)Binding & thermodynamicsEnzyme-inhibitor interactions
Surface Plasmon Resonance (SPR)Real-time binding kineticsDrug-enzyme studies

๐Ÿ“ฆ Applications

  • Clinical Diagnostics: Liver enzymes (ALT, AST), amylase, lipase

  • Food Industry: Protease, amylase, lactase assays

  • Pharmaceuticals: Enzyme inhibitors and drug development

  • Environmental: Enzyme biosensors for pollutant detection


๐ŸŽ“ Learn & Practice

  • Use model enzymes like catalase, peroxidase, urease, amylase

  • Perform lab-based activity assays + calculations of enzyme units, specific activity

  • Ideal for B.Sc./M.Sc. project work, internships, or lab-based job roles

Molecular Biology Training

 


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Mode: Offline/Online

๐Ÿงฌ Molecular Biology Training Overview

Molecular Biology Training focuses on understanding and manipulating the molecular mechanisms of life—primarily DNA, RNA, and protein synthesis and regulation. It is essential for careers in biotechnology, medical research, diagnostics, forensic science, and genetic engineering.


๐Ÿ” Core Training Topics

  1. DNA & RNA Techniques

    • DNA/RNA extraction & quantification

    • Gel electrophoresis (agarose, PAGE)

    • PCR, RT-PCR, qPCR

    • cDNA synthesis, RNA interference

  2. Cloning & Recombinant DNA Technology

    • Plasmid isolation

    • Restriction digestion and ligation

    • Transformation (E. coli)

    • Blue-white screening

    • Vector design and gene expression

  3. Protein Analysis

    • SDS-PAGE and Western blotting

    • Protein purification (chromatography)

    • ELISA (Enzyme-Linked Immunosorbent Assay)

    • Immunoprecipitation

  4. Gene Editing & Advanced Techniques

    • CRISPR-Cas9 basics

    • DNA microarrays

    • Next-Generation Sequencing (NGS) introduction

    • Bioinformatics for molecular biology

  5. Lab Safety and Good Laboratory Practice (GLP)

    • Aseptic techniques

    • Biosafety levels

    • Proper use of micropipettes and lab equipment


๐Ÿงช Hands-On Skills You’ll Gain

  • Designing primers for PCR

  • Running and analyzing electrophoresis gels

  • Preparing competent cells and performing transformation

  • Quantifying DNA/protein using spectrophotometers or fluorometers

  • Troubleshooting PCR and cloning experiments


๐ŸŽ“ Ideal For

  • B.Sc./M.Sc. students in Biotechnology, Microbiology, Biochemistry, Genetics

  • Professionals preparing for research or Ph.D.

  • Aspirants for molecular diagnostics, genetic counseling, or biotech industry roles


๐Ÿซ Where to Get Training (India-specific options)

  • ITLS Academy


๐Ÿ“ˆ Career Outcomes After Training

  • Research Assistant / Junior Research Fellow (JRF)

  • Biotech/Biopharma R&D roles

  • Clinical molecular diagnostics labs

  • Ph.D. positions in India or abroad

  • Forensic labs / Genetic testing centers

Clinical Microbiology


REGISTER NOW

Mode: Offline/Online

Clinical Microbiology is a critical branch of medical microbiology that focuses on the detection, identification, and study of microorganisms (like bacteria, viruses, fungi, and parasites) that cause human diseases. It plays a vital role in diagnosing infectious diseases, guiding treatment decisions, and supporting public health surveillance.


๐Ÿ”ฌ Key Areas of Clinical Microbiology

  1. Diagnostic Microbiology

    • Identification of pathogens from clinical specimens (blood, urine, sputum, CSF, etc.)

    • Culture techniques, microscopy, biochemical tests

  2. Antimicrobial Susceptibility Testing (AST)

    • Determining the resistance or sensitivity of microorganisms to antibiotics

    • Methods: Kirby-Bauer disk diffusion, MIC testing, E-tests

  3. Molecular Diagnostics

    • PCR, RT-PCR, nucleic acid probes for rapid and specific detection

    • Used for TB, COVID-19, STDs, etc.

  4. Serology and Immunology

    • Detection of antibodies or antigens in patient samples

    • Used for viral infections (HIV, Hepatitis B/C, Dengue, etc.)

  5. Infection Control and Hospital Epidemiology

    • Monitoring and preventing nosocomial (hospital-acquired) infections

    • Surveillance and outbreak investigation

  6. Parasitology and Mycology

    • Diagnosis of diseases caused by parasites and fungi (e.g., malaria, candidiasis)


๐Ÿฅ Clinical Microbiologist Role

  • Work in clinical labs in hospitals, diagnostic centers, and research institutions

  • Collaborate with physicians to interpret lab results and advise on treatment

  • Involved in infection control programs, vaccine development, and epidemiological tracking


๐Ÿงช Tools & Techniques Used

  • Gram staining, Ziehl-Neelsen staining (AFB)

  • Culture media (MacConkey, Blood agar)

  • ELISA, Western blot

  • PCR, qPCR, LAMP

  • MALDI-TOF mass spectrometry

  • Automated systems (VITEK, BACTEC)


๐ŸŽ“ Career Path & Skills

Educational Background:

  • B.Sc. or M.Sc. in Microbiology/Medical Lab Technology

  • Specialized diploma or Ph.D. for advanced roles

  • Clinical internship/training is often essential

Skills Needed:

  • Laboratory and diagnostic skills

  • Knowledge of biosafety and aseptic techniques

  • Interpretation of microbiological data

  • Attention to detail, communication skills


๐Ÿ” Applications & Importance

  • Disease diagnosis and surveillance

  • Antibiotic resistance monitoring (e.g., MRSA, ESBL)

  • Public health interventions

  • Vaccine development and efficacy monitoring

  • Outbreak investigation and control

 

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PATHOLOGY LAB ASSISTANT

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