Molecular Biology Training

 

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🧬 Molecular Biology Training Overview

Molecular Biology Training focuses on understanding and manipulating the molecular mechanisms of life—primarily DNA, RNA, and protein synthesis and regulation. It is essential for careers in biotechnology, medical research, diagnostics, forensic science, and genetic engineering.

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🔍 Core Training Topics

1. DNA & RNA Techniques

   • DNA/RNA extraction & quantification

   • Gel electrophoresis (agarose, PAGE)

   • PCR, RT-PCR, qPCR

   • cDNA synthesis, RNA interference

2. Cloning & Recombinant DNA Technology

   • Plasmid isolation

   • Restriction digestion and ligation

   • Transformation (E. coli)

   • Blue-white screening

   • Vector design and gene expression

3. Protein Analysis

   • SDS-PAGE and Western blotting

   • Protein purification (chromatography)

   • ELISA (Enzyme-Linked Immunosorbent Assay)

   • Immunoprecipitation

4. Gene Editing & Advanced Techniques

   • CRISPR-Cas9 basics

   • DNA microarrays

   • Next-Generation Sequencing (NGS) introduction

   • Bioinformatics for molecular biology

5. Lab Safety and Good Laboratory Practice (GLP)

   • Aseptic techniques

   • Biosafety levels

   • Proper use of micropipettes and lab equipment

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🧪 Hands-On Skills You’ll Gain

• Designing primers for PCR

• Running and analyzing electrophoresis gels

• Preparing competent cells and performing transformation

• Quantifying DNA/protein using spectrophotometers or fluorometers

• Troubleshooting PCR and cloning experiments

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🎓 Ideal For

• B.Sc./M.Sc. students in Biotechnology, Microbiology, Biochemistry, Genetics

• Professionals preparing for research or Ph.D.

• Aspirants for molecular diagnostics, genetic counseling, or biotech industry roles

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🏫 Where to Get Training (India-specific options)

• ITLS Academy

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📈 Career Outcomes After Training

• Research Assistant / Junior Research Fellow (JRF)

• Biotech/Biopharma R&D roles

• Clinical molecular diagnostics labs

• Ph.D. positions in India or abroad

• Forensic labs / Genetic testing centers